New Directions in Electrophoretic MethodsJames W. Jorgenson, Marshall Phillips Annotation Developed from a symposium at the 190th Meeting of the American Chemical Society, Chicago, Illinois, September 8-13, 1985. Electrophoresis remains the most powerful method available for separating and analyzing complex mixtures of charged biopolymers, playing a key role in the latest advances in medicine, agriculture, chemistry, and biotechnology. This sixteen-chapter volume traces recent developments in electrophoretic separations, technology, and applications. Isotachophoresis, zone electrophoresis, isoelectric focusing, two dimensional electrophoresis, and pulsed electrophoresis are among the methods discussed. Additional topics include applications of electrophoretic methods in agriculture and forensic serology, the development of standards for electrophoresis, and synthetic ion-containing polymers. Annotation(c) 2003 Book News, Inc., Portland, OR (booknews.com). |
Contents
Electrophoresis in Polyacrylamide Gels | 20 |
Recent Developments | 33 |
Rehydratable Polyacrylamide Gels for UltrathinLayer | 54 |
Copyright | |
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Common terms and phrases
2-D electrophoresis acrylamide acrylate addition agarose gel agarose gel electrophoresis amino acids ampholytes Anal analysis analytical Anderson applied bands basic amino acids Biochem Biophys Budowle buffer capillary carrier ampholytes charge Chem Chemical Chromatogr chromosomes color components concentration containing copolymer counterion density detection detector developed DNA molecules electric field electrode electrolyte electropherogram Electrophoresis 1985 electrophoretic mobility Elsevier Everaerts field strength Figure fractions gel electrophoresis genetic Gianazza glycerol groups hemoglobin Immobiline increased ionic IPG gel isoelectric focusing isoelectric point Isotachopherograms Isotachophoresis Laboratory leading electrolyte linear marker membrane method migration mixture molecular weight obtained P.G. Electrophoresis particles patterns permission from Ref pH gradient polyacrylamide gels polymer polymerization potential preparative procedure produce pulsed electrophoresis quantitative Radola ratio reagents rehydratable gels rehydrated Reproduced with permission resolution Righetti sample sensitivity sieving silver staining solution standards technique temperature tube two-dimensional ultrathin-layer voltage gradient zone electrophoresis