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Intracellular Distribution of Steroid Hormone
The Acceptor Hypothesis
28 other sections not shown
acceptor adsorbed adsorption Aliquots assay binding curves binding sites Biol buffer containing centrifuged Chapter Chem conformational change cooperativity cytosolic receptor diethylstilbestrol dilution dimer dissociation kinetics dissociation rate dithiothreitol DNA binding proteins DNA-cellulose EDTA eluted equilibrated equilibrium binding estimated estradiol estradiol binding estrogen receptor Figure fractional saturation guanidine-HCl H]estradiol H]estradiol-receptor complexes heat-transformed high affinity Hill coefficient hormone binding hydroxylapatite incubated indicate intact cells intracellular ionic strength isologous Jensen Katzenellenbogen labeled ligand mechanism molecular weight monomeric monomers nitrocellulose nitrocellulose filter nonspecific binding nuclear fraction nuclear receptor nuclei oligomer oligomeric pituitary progesterone receptor prolactin purified receptor quaternary structure receptor aggregates receptor concentration receptor forms receptor monomers receptor proteins receptor transformation Redeuilh renaturation samples Scatchard plots Sica and Bresciani similar specific binding steroid receptors subunit interactions supernatant temperature tetramer theoretical curves tissues Toft transformed receptor Tris-HCl uterine cytosol vitro washed Weichman and Notides Williams and Gorski Yamamoto and Alberts