Phenotypic Characterizations of C3H/10T1/2 Cl 8 and Its Transformed Variants: Isolation of Mutants Temperature-sensitive for Expression of the Transformed State from Chemically Transformed C3H/10T1/2 Cl 8 Cells |
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ability Acad acetone activator agar agarose alterations anchorage independence appear assay C1 16 cells C1 8 cells Cancer Res carcinogenesis cell culture cell culture systems cell lines cellular changes characteristics chemical carcinogens chemically transformed clones colonies concentration confluent correlates described determined developed dishes DMBA doubling effects efforts embryo cells enhanced examined exhibit experiments expression fibroblasts Figure foci formation formed FUdR gene grow grown growth hamster Heidelberger human increased incubation induced inhibition initiation isolated malignant medium Methods methyl cellulose morphology mouse embryo mutants Nature nontransformed observed obtained oncogenic transformation passages phenotype phorbol plastic plating efficiency presence Proc processes production properties reported requirements saturation density seeded selection serum showed shown studies suggests surface Table temperature temperature-sensitive tested trans transformed cells treated treatment tumor promoters tumorigenicity Type variants virus vitro