The Use of Reversible Chemical Crosslinking Reagents in Studies on the Structure of Viruses |
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Contents
III | 57 |
B A Study of RAV61 Structure Based on Analysis | 63 |
An Analysis of ReductantReversible Crosslinking | 76 |
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2-mercaptoethanol 30 minutes 3H]leu Mahoney polio acid added addition allowed to proceed analysis apparent arginine associations avian bonds bottom BSOCOES crosslinking buffer cleavage concentration containing crosslink reversal crosslinked complexes crosslinking reaction crosslinking reagent described determined dimer disulfide DMSO effect electro ester experiments exposure extent Figure final fractions function gel profile gradients higher identified intact Mahoney polio particles Mahoney polio virions major marker material minutes at 20°C mM BSOCOES mobility mock-crosslinked molecular weight observed obtained occur panel peak periodate placed polyacrylamide SDS tube polypeptide associations polypeptides portions possible preparation procedures proteins RAV-61 virion reduced reversal RNA tumor sample samples were subjected SDS slab gel SDS tube gels sedimentation seen shown slab gel sodium phosphate standard stopped stored strip structure studies subjected to electrophoresis sucrose Table trimer two-dimensional virus viruses