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0.0l M Tris-HCl 22S and l5S 30S particle 30S proteins 30S ribosomal proteins 30S subunits acetic acid acrylamide analysis of l6S binding sites centrifugation chemical modification containing core particles crosslinked determine dialysis dialyzed digested with Enzite Enzite RNase enzyme extracted Fellner fractions heated histidine hours at 42°C identification incorporation of iodine incubation iodinated at 42°C iodination of l6S iodination reaction iodine incorporated ionic strength isolated l5 minutes l5S fragment l6S RNA iodinated l6S RNA molecule magnesium nitrocellulose filter Nomura nuclease digestion nuclease fragments nucleotides oligonucleotides phenol precipitated protein composition protein S4 protein-RNA complexes protein-RNA interactions proteins associated proteins present reagent reconstitution buffer regions of l6S ribonuclear-protein ribonuclease ribosomal RNA RNA binding RNA conformation RNA fragments rRNA sample secondary structure Sedimentation Sephadex sequence shown in Figure specific subparticles sucrose gradient profiles Table l2 temperature terminus tertiary structure TlCl Tris-HCl pH 8.0 two-dimensional paper electrophoresis unpaired cytosines urea Zimmermann