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single stranded DNA
Function and Antitermination by Phage Lambda Q Protein
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1mM DTT 35 element a70 mutants a70 subunit addition amino acids antitermination arabinose Borukhov buffer Burgess cells cleavage factors cleaves DNA coli RNA polymerase conformational change conserved region core enzyme crosslinking DNA binding downstream early transcribed region EDTA effect elongation complex elongation factors enzyme Escherichia coli Escherichia coli RNA FeBABE conjugated gene Gre factors Gre proteins GreA and GreB greA/B half-life helix holoenzyme holoenzyme containing induces the pause interaction Kainz and Roberts KMn04 footprinting Krummel and Chamberlin lambda Q lanes mutant mutant a70 mutant sigma nontemplate strand nucleotides Nudler oligo oligonucleotide open complex open promoter complex operon pause inducing sequence paused complex plasmid polypeptide position promoter DNA promoter pR promoter recognition Q activity Q function Q modification reaction readthrough regulatory pause rifampicin RNAP rpoD Samples were removed sigma 70 sigma factors sigma subunit single stranded DNA stability strain structure synthesis template terminators upstream vitro transcription wild type wildtype