Organization of the Extracellular MatrixThis book describes analysis techniques and results of topics such as physical backgrounds, chemical backgrounds, and principal methods of topo-optical reactions used in ultrastructure research of the ECM; orientation patterns of GAGs and collagen in different tissues/cartilage, cornea, kidney basement membranes, and skin; factors involved in the formation of submicroscopically ordered matrix structure; and cell-matrix interactions, including cytoskeleton-cell-membrane-matrix relationships. A summarization of the advantages and limitations of polarization microscopy compared to electron microscopy in ultracellular research is also included. Cell biologists, histologists, pathologists, and biochemists in connective tissue research will find this book to be an invaluable reference tool. |
Contents
Introduction | 10 |
Operations with the Polarization Microscope | 25 |
TopoOptical Reactions Used in Polarization Microscopic Ultrastructure | 31 |
Chapter 4 | 61 |
Structure of the Hyaline Cartilage Matrix | 75 |
The Organic Bone Matrix | 99 |
Chapter 6 | 115 |
Chapter 7 | 137 |
Other editions - View all
Common terms and phrases
ABT reaction Acad Acta Histochem alcian blue amyloid fibrils Anat anionic articular cartilage basement membrane binding Biochem birefringence blue at pH bone matrix bovine cationic dyes Cell Biol cell membrane chondrocytes chondroitin sulfate chromotrope collagen fibers collagen fibrils collagen type collagen type IV congo red connective tissue constituents corneal stroma Cytochem dichroism digestion with testicular dimethylmethylene blue dye molecules electron microscopic embryonic extracellular matrix fibroblasts fibronectin FIGURE filaments glycoproteins glycosaminoglycans groups heparan sulfate Histochemistry human articular cartilage hyaluronidase induced interactions keratan sulfate lamellae lamina laminin long axis longitudinal metachromasia Módis mol/l molecular optical anisotropy orientation pattern orthochromatic osteocyte osteons perpendicular picrosirius red F3B polarization color polarization microscopic polarized light polyanions protein proteoglycans refractive index retardation values Romhányi sections sign of birefringence spatial orientation specimen structure sulfated GAGs technique territorial matrix testicular hyaluronidase Tissue Res toluidine blue topo-optical reactions ultrastructure X-ray zone