Principles and techniques of electron microscopy: biological applications

M. A. Hayat

Cambridge University Press, 2000 - Science - 543 pages

Principles and Techniques of Electron Microscopy has established itself as a standard book for biological electron microscopists wishing to learn how to prepare their specimens for electron microscopic investigation. This fully revised and expanded 4th edition includes three new chapters covering such topics as immunocytochemistry and applications of microwave irradiation to microscopy. This revised and expanded 4th edition of Principles and Techniques of Electron Microscopy provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. The book presents methods in a self-explanatory form. Further, alternative procedures and points of disagreement help the reader interpret data accurately. What sets this book apart from its competition is that it not only describes techniques but also explains the principles behind the techniques; that is, chemical reactions underlying the use of various reagents for preserving and staining cellular components.

What people are saying

User Review -

This book is excellent piece of work by Dr. M.A Hayat. Research techniques are discussed in detail and are presented in an efficient way. In addition the book presents all information about the reagents and solutions which required at varoius stages of processing. All information is available for beginners who are at the initial stages of their research. The information is concise and easy to comprehand. All the concepts and questions regarding electron microscopy are well answered after reading this book. As a parasitologist, working on SEM studies on helminth parasite find suitable for the beginners and research scholars who want to involve themselves in the field of electron microscopy based research.
Ummer Rashid Zargar
Ph D research scholar
CORD
University of Kashmir
Pin 190006 

User Review -

A must for students of electron microscopy. All of Hayat's books are classics and essential for the lab or library.

Contents

• Preface page xvii Phosphate Buffers: 25
• vii: 188
• OSMIUM TETROXIDE: 61
• CHAPTER THREE: 85
• POLYMERIZATION: 91
• LOWICRYLS: 109
• POLYETHYLENE GLYCOL CARBOWAX: 115
• SPECIMEN ORIENTATION: 121
• GRADUAL PROGRESSIVE DEHYDRATION: 127
• REEMBEDDING: 133
• GLASS KNIVES: 140
• SAPPHIRE KNIVES: 151
• SECTIONING ANGLES: 159
• PREPARATION OF TROUGHS: 166
• SECTION VIEWING: 174
• SECTIONING PROCEDURE: 182
• SECTION CONTAMINATION AND DAMAGE: 197
• ELECTRON MICROSCOPE TOMOGRAPHY: 205
• CHAPTER FIVE: 211
• CHAPTER: 242
• FACTORS AFFECTING CONTRAST: 248
• COLLOIDAL GOLD: 260
• Multiple Immunogold Staining Methods: 271
• DIAMINOBENZIDINEOSMIUM TETROXIDE: 281
• SILVER: 324
• SILVER LACTATEOSMIUM TETROXIDE: 334
• URANYL PREPARATIONS: 342
• Methyl Green and Methyl Violet: 366
• CHAPTER SEVEN: 367
• GENERAL METHODS: 377
• GENERAL METHODS FOR HUMAN: 389
• CHAPTER EIGHT: 400
• RATE OF COOLING: 406
• FREEZE DRYING 419 Cutin: 453
• LIMITATIONS OF LOWTEMPERATURE Immunogold Labeling of Pectin in Tissues: 460
• CELL WALL 440 Suberin: 466
• CHAPTER: 472
• MICROTOMY OF PARAFFIN SECTIONS: 479
• Epitope Retrieval with Enzyme Digestion: 490
• Index: 533

Bibliographic information

• Title: Principles and techniques of electron microscopy: biological applications
• Author: M. A. Hayat
• Editor: M. A. Hayat
• Contributor: M. A. Hayat
• Edition: 4, illustrated
• Publisher: Cambridge University Press, 2000
• ISBN: 0521632870, 9780521632874
• Length: 543 pages
• Subjects: Medical / Laboratory Medicine; Medical / Pathology; Science / Electron Microscopes & Microscopy; Science / Microscopes & Microscopy