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acinar cells acini activation aequorin agonist antibody basal Biophys buffer Ca2+ channels Ca2+ release Ca2+ stores Ca2+ waves Ca2+-ATPase calcium channels calcium concentration calcium inﬂux calcium release calcium signalling calcium waves calmodulin calpain calsequestrin cardiac Cell Biol Cell Calcium cellular cium cultured cytoplasmic cytosolic depolarization Dept Physiol effect EGTA ﬁbres ﬁbroblasts ﬁrst ﬂuorescence fraction free calcium increase incubated induced inhibition inhibitors inositol phosphate inositol trisphosphate InsP3 Inst intracellular intracellular Ca2+ intracellular calcium isolated Japan levels liver measured mechanism medium Molec mouse muscarinic neurons NEUROSCI neutrophils oocytes oscillations pancreatic acinar cells PDGF plasma membrane platelets propagation protein kinase puriﬁed receptor regulation response role sarcoplasmic reticulum secretion signiﬁcant smooth muscle cells speciﬁc sphingosine spikes stimulation studies thapsigargin thrombin tion tissue transients trisphosphate Univ uptake vesicles Xenopus oocytes