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adult cardiac fibroblasts antibody applied strain assayed for IGF-I autocrine/paracrine factors binding proteins Cardiac IGF-I cardiac myocytes cardiomyocytes Cardiovasc cell culture cellular chamber and indenter concentrations by ELISA conditioned media cyclic stretch device cytoskeleton endothelial cells equibiaxial exocytosis extracellular matrix factor-I fibroblasts were stretched Figure 11 focal adhesion G-proteins gene expression Gibco/Invitrogen heart hypertrophy IGF-I concentrations IGFBP induced IGF-I secretion Insulin-like growth factor integrins MAPK mechanical forces mechanical stimuli mechanical stretch mechanotransduction membrane holder mitogen-activated protein kinases modulate myocardial infarction myocardium myostatin non-stretched cardiac fibroblasts normalized to total paracrine signaling Physiol pmol/lE6 cells presented as means proliferation rat cardiac fibroblasts receptor regulation remodeling respond to mechanical secretion from cardiac secretion in cardiac secretion of IGF-I silicone membrane smooth muscle cells static stretch static stretch device stretch chamber stretch on IGF-I Stretch-activated ion channels stretch-induced IGF-I release Stretch-induced increases stretched cardiac fibroblasts total cell number tyrosine kinases ventricular verapamil vimentin