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acetone alcoholic extract allowed to evaporate amount of extract amyl alcohol analysis aqueous extract Arch ash by Morin ash content Ash determinations benedictus are given bitter principle bitter taste blessed thistle calcium malate calcium oxalate Canstatt's Jahresber Carduus Centaurea Chim chloroform cnicin obtained Cnicus benedictus crystalline material crystals of potassium crystals separated crystals were obtained digitaline dissolved distillation dried ether solution extract obtained extracted with petroleum Extractum Cardui benedicti fatty acids Fehling's solution Ferencz filtered filtrate flask formula Frickhinger 1853 Geiseler glucoside gram of cnicin heated heptane Herba Cardui benedicti insoluble iron oxide Journ large amount large number magnesium malate melting point methyl alcohol Nativelle nitric acid old extract percentage petroleum ether plant potassium carbonate potassium chloride potassium nitrate purified recrystallization Repertorium f. d. Pharm residue Rosenthaler salicin saponification Schwandner Scribe selective solvents small amount Soltmann soluble stems total ash treated Ueber weighed yielded