Gene Cloning: An Introduction |
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Page 16
... copy number refers to the number of molecules of an indi- vidual plasmid that are normally found in a single bacterial cell . The factors that control copy number are not well understood , but each plasmid has a characteristic value ...
... copy number refers to the number of molecules of an indi- vidual plasmid that are normally found in a single bacterial cell . The factors that control copy number are not well understood , but each plasmid has a characteristic value ...
Page 44
... copy number of a plasmid . Some multicopy plasmids ( those with copy numbers of 20 or more ) have the useful property of being able to replicate in the absence of protein synthesis . This contrasts with the main bac- terial chromosome ...
... copy number of a plasmid . Some multicopy plasmids ( those with copy numbers of 20 or more ) have the useful property of being able to replicate in the absence of protein synthesis . This contrasts with the main bac- terial chromosome ...
Page 111
... copy number than pBR322 , being present at about 30-45 molecules per E. coli cell . This is not of great rele- vance as far as plasmid yield is concerned , as both plasmids can be amplified to copy numbers greater than 1000. However ...
... copy number than pBR322 , being present at about 30-45 molecules per E. coli cell . This is not of great rele- vance as far as plasmid yield is concerned , as both plasmids can be amplified to copy numbers greater than 1000. However ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast