Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 77
Page 1-7
... restriction sites that could be used for cloning . The first plasmid to combine all of the then - available desirable features was pBR313 ( Bolivar et al . 1977a , b ) : It replicated in a relaxed fashion , contained two selectable ...
... restriction sites that could be used for cloning . The first plasmid to combine all of the then - available desirable features was pBR313 ( Bolivar et al . 1977a , b ) : It replicated in a relaxed fashion , contained two selectable ...
Page 1-59
... Restriction Sites in the Plasmid Vector and the Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the ...
... Restriction Sites in the Plasmid Vector and the Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the ...
Page 5-16
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml