Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 77
Page 1-42
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
Page 1-44
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
Page 1-78
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml