Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 97
Page 1-66
... vector DNA : foreign DNA . For most routine cloning purposes , this is unnecessary . The curves in Figures 1.9 , 1.10 , and 1.11 show that reasonable numbers of useful recombinants will be obtained when the ratio of plasmid DNA : foreign ...
... vector DNA : foreign DNA . For most routine cloning purposes , this is unnecessary . The curves in Figures 1.9 , 1.10 , and 1.11 show that reasonable numbers of useful recombinants will be obtained when the ratio of plasmid DNA : foreign ...
Page 2-82
... DNA sequences propagated in vectors with larger capacity ( e.g. , cosmids ) to construction of complex cDNA or genomic DNA libraries . Cloning in bacteriophage A ... Vectors Cloning in Bacteriophage λ 2 82 PREPARATION OF VECTOR DNA 2 82.
... DNA sequences propagated in vectors with larger capacity ( e.g. , cosmids ) to construction of complex cDNA or genomic DNA libraries . Cloning in bacteriophage A ... Vectors Cloning in Bacteriophage λ 2 82 PREPARATION OF VECTOR DNA 2 82.
Page 4-33
... DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector ... DNA is ligated with an ...
... DNA are inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector ... DNA is ligated with an ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml