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acid amino-acids Anat animal cells apparatus ascites aseptic autoclave balanced salt solution Biochem Biol blood Cancer Inst Cancer Res Carrel Cell Res cell strains cell suspension cells in tissue cent centrifuge chamber chick embryo chick heart chromosomes concentration containing coverslip cultivation cultured cells cystine developed dilute embryo embryo extract epithelial essential explants fibroblasts filters flask fluid glass glassware glucose glutamine grow grown growth growth medium human incubator laboratory large number mammalian cells material medium membrane metabolism method microscope minutes mitosis morphological mouse N.Y. Acad normal obtained organ culture osmotic pressure Pasteur pipette Petri dish Physiol pipette plant tissue culture plasma clot poliomyelitis prepared Proc protein removed Rinse serum slide sodium sterile sterilised stock solution stoppers studies survival synthetic technique temperature test-tubes Thymine tion tissue culture transferred transplantation trypsin tube tumour vessel Virology virus viruses vitamin vitro washed