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aberration acid adjustment angle apochromatic apparatus back lens beam of light Bellows length blue body tube camera Canada balsam cells centre chromatic chromatic aberration colour temperature correct cover glass crystals dark-ground depth of field draw tube emulsion eyepiece film filter fitted flash fluorescence fluorite focal length focus focusing give grain green haematoxylin high-power illumination illustrated immersion infra-red interference iris diaphragm lamphouse Leitz lenses light-source lower magnification medium method micro microscope microtome minutes mirror mounted necessary negative normal numerical aperture oblique oil-immersion optical axis optical system panchromatic photographed photomicrographs placed plane plate polarized light position prism produce quartz radiations rays reflector refractive index resolving power seconds seen shown in Fig silver slide specimen spherical aberration stained substage condenser suitable surface technique thickness total x total x 400 transmitted light transparent Tube length ultra-violet ultra-violet radiation vertical Watson wave-length Wratten