The Scaffolding Molecule, GAB2, is a Key Regulator in Neural Stem Cell Biology and Hematopoietic Cell SignalingUniversity of Michigan., 2005 |
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32D cells AKT activation anti-Gab2 apoptosis assay B-III tubulin bFGF bFGF-mediated binding sites catalog cDNA cell line cell number cell proliferation cell survival cell viability clone 24 control or Gab2 coverslips downstream embryonic ERK activation ERK pathway FGFR FRS2 functions Gab proteins Gab1 Gab2 and Gab3 Gab2 expression Gab2 is required Gab2 mutants Gab2 silencing Gabl gene Grb2 growth factor growth medium Hoechst inhibitor insulin interaction Invitrogen laminin lentivirus levels luciferase mediated membrane minutes neural stem cells neurogenesis neuronal neuronal differentiation neurospheres ng/ml NSCs oligodendrocyte overexpression P19 cells p85 binding PH domain phosphorylation PI3K PIP3 primer RA-induced cell death RasGAP receptor regulation response role of Gab2 RT-PCR scaffolding proteins secondary antibodies SH2 domain SHP2 shRNA constructs shRNA-2 siGab2 clone signaling pathways slice culture specific stable Gab2 staining stimulation Total cell transduced transfected tubulin tyrosine phosphorylation vector vitro Western blotting WT-Gab2 µg/ml