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30 minutes acetic acid adsorption average bacterial associate bacterial streak Bonner cell ploidy cellular slime molds centrifugation coli coli B/r concentration crossed streaks curve was prepared cycle deoxyribonucleic acid determined diameter of spores difference diphenylamine diploid diploid populations discoideum was grown division elliptical extract Feulgen's Frequency distribution g Bacto-agar Germination rate growth heat shock incubated inoculated inoculum investigation isolate showing large celled isolate large celled population large cells large myxamoebae large spores liquid culture Macromolecular Composition major diameter measured ml of cold morphogenesis Myxamoebae emerged newly emerged myxamoebae non-nutrient agar nuclei nucleic acid observed optical density phase contrast phosphate buffer placed on non-nutrient ploidal polyploid procedure pseudoplasmodium Raper reagent sample small myxamoebae small spores sori sorocarp formed sorus spores formed stain standard curve stock cultures studies Sudan black Sussman and Sussman Sussman l962 Swollen spores synthetic medium vegetative