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LargeScale Gradient Elution Chromatography
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adsorption affinity chromatography affinity interactions affinity ligand affinity partitioning affinity precipitation affinity ultrafiltration AIChE Albertsson P-A ammonium sulfate antibody aqueous two-phase systems binding Biochem biomolecule Biotechnol Bioeng Biotechnology bottom phase Chem Chromatogr column length complex Dehydrogenase detailed model developed dextran diffusion effect eluates eluent eluent concentration enzyme extract filtration Flory-Huggins theory flowrate flux gradient elution chromatography gradient period gradient shape hollow fiber hydrophilic hydrophobic immobilized increased ion exchange ionic strength Johansson G Kula M-R ligand linear gradient elution Linear Linear Linear lysozyme macroligand mass transfer membrane bioreactors mixer mixing mobile phases molecular weight molecules myoglobin particles partition coefficient Partitioning in Aqueous PEG/dextran system phase diagram phase forming polymer phase system phosphate polyelectrolyte polymer polymer concentration pore protein partition purification reactor recovery result Shanbhag shown in Fig soluble solution solvent substrate surface target protein technique temperature top phase Triazine Triazine Dye trypsin ultrafiltration