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Development and Evaluation of a Protocol
Development of a Novel HelperDependent Adenovirus
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adenovirus vectors assayed BGHpA bioluminescent imaging cell line cells were infected CFP expression chromosomes CMV promoter coinfection Cre recombinase Cre-mediated cytosines D17 cells diluted Dorigo EBNA1 EBV episome Epstein-Barr virus experiments expression cassette EYFP F. L. Graham fluorescence Forward Primer fraction Gene Ther gene therapy gene therapy vectors genetic genomes HDA-EBV hybrid system HDA.CFP+FR HDA.Cre HDAd vectors HDAd-EBV hybrid vector HDAd-EBV vector HDAd-EBV.RL HDAd-EBV.RL+FR HDAd.RL Helper Dependent Adenovirus helper virus helper-dependent hepatocytes hybrid vector infected cells integration linear vector liver tissue maintained maintenance mammalian cells methylation mice Molecular mouse number of cells OriP plasmid primer set primer/probe set probe protein protocol puromycin qPCR Renilla luciferase replication Reverse Primer RL expression sample sequences stable target cells target vector titers titration Total DNA total number transduced transgene expression vector DNA vector genomes vector stock vector system viral Virol vitro vivo week