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acid per ml aged 24 hour aliquots Analysis of Variance ascorbic acid autoxidation boar semen boar spermatozoa bull spermatozoa Caffier Commercial Lipid cytochrome decrease density of TBA df MSC df diluted semen stored effect of hydrogen Effect of Storage effects of spermatozoa embryo survival embryonic development embryonic mortality Extended and Unextended Extracted Semen Lipid farrowing rate Horwitt hour controls hours resulted hydrogen peroxide increased Incubated and Treated incubation at 37 insemination investigators levels of hydrogen linolenic linolenic acid lipid peroxidation lipids in boar lipoprotein complex Mann Mean Effect mean optical density Mean Square membrane mitochondria MSa df optical density values oxidation peroxidation occurred Peroxide on Optical pro-oxidants red blood cells reproductive tract round bottom flask Salisbury semen age semen was stored senescence Sidwell significantly more peroxidation spermatozoa age spermatozoon cell steam distillation Storage and Hydrogen subsamples sulfhydryl groups TBA Reactants TBA reactive material TBA test test for lipid