Gene Therapy Protocols: Volume 2: Design and Characterization of Gene Transfer Vectors

Front Cover
Joseph LeDoux
Humana Press, Apr 24, 2008 - Science - 313 pages
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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

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Contents

ChromatographyBased Purification of AdenoAssociated Virus
37
Spectroscopic Methods for the Physical Characterization
55
RealTime Multiple Particle Tracking of Gene Nanocarriers
81
Production of Lentiviruses Displaying EarlyActing Cytokines
99
Fibermodified Adenoviruses for Targeted Gene Therapy
113
PEGylated Adenovirus for Targeted Gene Therapy
133
TransposonBased Mutagenesis Generates Diverse
161
Photochemical Enhancement of DNA Delivery by EGF Receptor
171
Evaluation of Promoters for Use in TissueSpecific Gene Delivery
205
AdenovirusMediated Transduction of Auto and DualRegulated
221
LiverDirected Gene Therapy Using the Sleeping Beauty
267
Generation and Functional Analysis of Zinc Finger Nucleases
277
Conditional Gene Expression and Knockdown Using Lentivirus
291
Index
311
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