Gene Therapy Protocols: Volume 2: Design and Characterization of Gene Transfer Vectors, Volume 2; Volume 434
Humana Press, Apr 24, 2008 - Medical - 314 pages
In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). In this second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors.
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ChromatographyBased Purification of AdenoAssociated Virus
Spectroscopic Methods for the Physical Characterization
RealTime Multiple Particle Tracking of Gene Nanocarriers
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adeno-associated virus adenoviral vectors adenovirus agarose aliquot analysis antibody assay buffer capsid cell line centrifuge chromatography clones column complexes concentration containing CsCl cytokines digestion dilution DMEM elution FGF2 fiber filter filtration fluorescence fractions gene delivery gene expression Gene Ther gene therapy gene transfer genetic genome hematopoietic human incubate infection inserted Invitrogen lentiviral lentiviral vectors luciferase lysate medium membrane Methods mice Molecular mouse murine NaCl Note particles PEGylation pellet photochemical plasmid plasmid DNA plate polyplexes primers promoter protein protocol purification Qiagen QPCR reaction reagent receptor recombinant resuspend retroviral vectors room temperature sample sequence serotype solution stem cells sterile Subheading supernatant target therapeutic titer transcription transduction transfection Transfer Vectors transgene transgene expression transposase transposon Tris-HCl tube ug/ml viral viral vectors Virol viruses vitro vivo volume wash Western blot zinc finger