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12SI-labeled 3T3 cells Acad affinity amino acid amino acid sequence analysis antigen assay binding Biol biological activity blot bovine IL-2 cDNA clones cDNA library cell lines cell surface cellular characterization clone coli containing culture cytotoxic detected encoding expression vector fibroblast function gene genomic Gillis GM-CSF growth factor homology HuTNF-a hybridized IgE-binding factor IL-2 receptor IL-2-dependent immune Immunex Immunex Corporation Immunol induced interferon interleukin isolated lane Leu Leu leukocyte lymphocytes lymphokine Lymphotoxin macrophages mature medium Molecular Biology molecules monoclonal antibody monocytes mouse mRNA murine murine IL-2 receptor Natl nucleotide peptide plasmid polypeptide probe Proc produced proliferation protein purified recombinant region residues SDS-PAGE specific stimulation supernatants synthetic T-cell tion transcription transfected tumor necrosis Urdal vitro yeast Yodoi