Phytochemical Methods A Guide to Modern Techniques of Plant Analysis
This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.
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45 Sulphur compounds
52 Amino acids
55 Cyanogenic glycosides
24 Flavonoid pigments
26 Flavonols and flavones
27 Minor flavonoids xanthones and stilbenes
29 Quinone pigments
32 Essential oils
33 Diterpenoids and gibberellins
34 Triterpenoids and steroids
42 Fatty acids and lipids
43 Alkanes and related hydrocarbons
57 Purines pyrimidines and cytokinins
64 Sugar alcohols and cyclitols
72 Nucleic acids
A list of recommended TLC systems for all major classes of plant chemical
Some useful addresses
absorbance absorption Academic Press acetic acid acetone acid-water aglycones alcohol alkaloids amines amino acids analysis anthocyanins aqueous aromatic Biochemistry blue buffer carotenoids cellulose chemical Chemistry and distribution chloroform chlorophylls column common compounds concentrated constituents cyanidin detected dried electrophoresis eluted enzyme essential oils esters ethanol ether example fatty acids filtered flavones flavonoids flavonols fluorescent fraction gibberellins glucose glycosides Harborne higher plants HPLC hydrolysis identification isolation lactones leaf lipids London markers measurements methods methyl mixtures NH4OH nucleic acids occur oligosaccharides paper petroleum phenolic phytochemical Phytochemistry pigments plant extracts plant tissues polyacetylenes polysaccharides present procedure protein purified quercetin quinones reagent Recommended techniques residue RF values sample seed separation silica gel silica gel plates simple sodium soluble solution solvent species spectral spectroscopy spots spraying structures substances sugar Table tannins terpenes terpenoids Thin layer chromatography TLC on silica UV light yellow
Page 183 - Baker EA, 1982. — Chemistry and morphology of plant epicuticular waxes. In The Plant Cuticle, DF Cutler, KL Alvin and CE Price, ed., Academic Press, London, 139-165.
Page 183 - In: Recent Advances in the Chemistry and Biochemistry of Plant Lipids, eds.
Page 183 - Harwood, JL (1980). Plant acyl lipids: Structure, distribution and analysis.