Phytochemical Methods A Guide to Modern Techniques of Plant Analysis

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Springer Science & Business Media, Apr 30, 1998 - Nature - 302 pages
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This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.
  

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Contents

Methods of plant analysis
1
12 Methods of extraction and isolation
4
13 Methods of separation
7
14 Methods of identification
16
15 Analysis of results
30
16 Applications
32
Phenolic compounds
40
22 Phenols and phenolic acids
42
44 Polyacetylenes
174
45 Sulphur compounds
179
Nitrogen compounds
187
52 Amino acids
189
53 Amines
198
54 Alkaloids
203
55 Cyanogenic glycosides
214
56 Indoles
218

23 Phenylpropanoids
49
24 Flavonoid pigments
60
25 Anthocyanins
66
26 Flavonols and flavones
74
27 Minor flavonoids xanthones and stilbenes
83
28 Tannins
90
29 Quinone pigments
96
The terpenoids
107
32 Essential oils
110
33 Diterpenoids and gibberellins
124
34 Triterpenoids and steroids
129
35 Carotenoids
138
Organic acids lipids and related compounds
151
42 Fatty acids and lipids
159
43 Alkanes and related hydrocarbons
170
57 Purines pyrimidines and cytokinins
222
58 Chlorophylls
227
Sugars and their derivatives
235
62 Monosaccharides
236
63 Oligosaccharides
244
64 Sugar alcohols and cyclitols
249
Macromolecules
256
72 Nucleic acids
257
73 Proteins
265
74 Polysaccharides
278
A list of recommended TLC systems for all major classes of plant chemical
291
Some useful addresses
294
Index
295
Copyright

Common terms and phrases

Popular passages

Page 183 - Baker EA, 1982. Chemistry and morphology of plant epicuticular waxes. In The Plant Cuticle, DF Cutler, KL Alvin and CE Price, ed., Academic Press, London, 139-165.
Page 289 - The extraction and purification of enzymes from plant tissues in Regulation of Enzyme Synthesis and Activity in Higher Plants (ed. H. Smith), Academic Press, London, pp.
Page 183 - In: Recent Advances in the Chemistry and Biochemistry of Plant Lipids, eds.
Page 183 - Harwood, JL (1980). Plant acyl lipids: Structure, distribution and analysis.
Page 288 - DE (1968) Chemosystematics with emphasis on systematic serology in Modern Methods in Plant Taxonomy (ed.

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About the author (1998)

J. B. Harborne is Professor of Botany, University of Reading, UK.

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