Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery (Google eBook)

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CRC Press, Nov 1, 2005 - Science - 528 pages
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The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information.

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays.

Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.
  

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Contents

Introduction
1
Micromachining Methods
3
212 BONDING OF Si CHIPS
6
22 MICROMACHINING OF GLASS
7
221 ETCHING OF GLASS MICROCHANNELS
8
222 DRILLING OF GLASS FOR ACCESSHOLE FORMATION
14
223 GLASS BONDING
16
23 MICROMACHINING OF FUSED QUARTZ OR FUSED SILICA
18
Detection Methods
187
7112 Scanning Detector
190
7113 Background Reduction
191
7114 Photobleaching Effect
192
7115 Integrated Fluorescent Detector
193
712 INDIRECT FLUORESCENT DETECTION
195
713 MULTIPLEPOINT FLUORESCENT DETECTION
196
714 ABSORBANCE DETECTION
200

24 MICROMACHINING OF POLYMERIC CHIPS
20
242 INJECTION MOLDING
29
243 ABLATION
30
244 WIRE IMPRINTING
33
245 COMPRESSION MOLDING
34
246 PHOTOPOLYMERIZATION
37
247 OTHER PLASTIC MICROMACHINING PROCESSES
38
248 PROBLEMS ENCOUNTERED IN POLYMERIC CHIPS
40
2482 Electrical Properties
42
2585 Surface Properties
43
2486 SolventResistant Properties
45
25 METAL PATTERNING
46
26 WORLDTOCHIP INTERFACE
49
Microfluidic Flow
55
312 PRESSUREDRIVEN FLOW
56
313 CENTRIFUGAL PUMPING
57
314 ALTERNATIVE PUMPING PRINCIPLES
59
3143 Thermally Induced Pumping
63
3144 Surface Energy
65
3147 Evaporation
66
315 MICROFLUIDIC FLOW MODELING STUDY
67
32 MICROFLUIDIC FLOW CONTROL
68
322 LAMINAR FLOW FOR LIQUID EXTRACTION AND MICROFABRICATION
72
323 GENERATION OF CONCENTRATION AND TEMPERATURE GRADIENTS
76
324 FLOW SWITCHING
79
325 FLUID MIXING
90
3251 Diffusive Mixing
91
3253 Oscillating Flow
95
3254 Acoustic Mixing
96
3255 Other Mixing Methods
98
326 LIQUID DISPENSING
99
A Sample Introduction
103
411 PINCHED INJECTION
107
412 GATED INJECTION
115
42 HYDRODYNAMIC INJECTION
117
43 OTHER SAMPLE INJECTION METHODS
121
Sample PreConcentration
123
52 EXTRACTION
125
53 POROUS MEMBRANE
129
54 OTHER PRECONCENTRATION METHODS
137
Separation
141
62 CAPILLARY ELECTROPHORESIS CE
143
622 CAPILLARY GEL ELECTROPHORESIS CGE
151
623 MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY MEKC
155
624 ISOTACHOPHORESIS ITP
158
625 CAPILLARY ELECTROCHROMATOGRAPHY CEC
160
626 SYNCHRONIZED CYCLIC CAPILLARY ELECTROPHORESIS SCCE
166
627 FREEFLOW ELECTROPHORESIS FFE
170
628 DERIVATIZATIONS FOR CE FOR SEPARATIONS
171
6282 PostColumn Derivatization
174
63 CHROMATOGRAPHIC SEPARATIONS
175
64 COUPLED SEPARATIONS
176
642 TWODIMENSIONAL SEPARATIONS
179
715 PLASMA EMISSION DETECTION
202
716 CHEMILUMINESCENCE CL DETECTOR
205
717 REFRACTIVE INDEX Rl
208
718 THERMAL LENS MICROSCOPE TLM
209
719 RAMAN SCATTERING
210
7110 SURFACEPLASMON RESONANCE
211
721 AMPEROMETRIC DETECTION
212
722 VOLTAMMETRIC DETECTION
218
723 POTENTIOMETRIC DETECTION
220
73 MASS SPECTROMETRY MS
224
732 MATRIXASSISTED LASER DESORPTION IONIZATION MALDI
235
74 OTHER DETECTION METHODS
238
742 ACOUSTIC WAVE DETECTION
246
743 NUCLEAR MAGNETIC RESONANCE NMR
247
Applications to CellularParticle Analysis
251
812 WEIRTYPE FILTERS
255
813 CELL ADHESION
263
814 POLYMER ENTRAPMENT
267
815 THREEDIMENSIONAL FLOW CONTROL
269
816 OPTICAL TRAPPING OF CELLS
273
817 DIELECTROPHORESIS DEP
277
82 STUDIES OF CELLS IN A FLOW
280
83 OTHER CELL OPERATIONS
288
832 ELECTROPORATION
290
833 CHIP CLEANING AND STERILIZATION
292
Applications to Nucleic Acids Analysis
293
92 NUCLEIC ACIDS AMPLIFICATION
294
9212 Surface Passivation of PCR Chambers
301
9214 RealTime PCR
306
9215 FlowThrough PCR
309
9216 Other DNA Amplification Techniques
310
922 RNA AMPLIFICATION
311
93 DMA HYBRIDIZATION
313
932 MICROARRAY DNA HYBRIDIZATION
317
942 GENETIC ANALYSIS
320
943 SEPARATION OF LARGE DNA MOLECULES
325
Applications to Protein Analysis
337
1012 HETEROGENEOUS IMMUNOASSAY
343
102 PROTEIN SEPARATION
349
103 ENZYMATIC ASSAYS
353
1032 ASSAY OF ANALYTES AFTER ENZYMATIC REACTIONS
358
Appendix
365
Problem Sets
393
3 MICROFLUIDIC FLOW
395
4 SAMPLE INTRODUCTION
396
6 SEPARATION
397
8 CELLULAR ANALYSIS
400
10 PROTEIN ANALYSIS
401
References
403
Glossary
477
Index
487
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