Gene Therapy Protocols, Volume 433Joseph M. Le Doux Humana Press, 2008 - Gene therapy Gene Therapy Protocols, Volumes 1 and 2 present a comprehensive collection of detailed methods and protocols used by the leaders in the field of gene therapy. |
Contents
Production of Retroviral Vectors for Clinical Use | 17 |
Methods for the Production of HelperDependent Adenoviral | 33 |
Vectors | 55 |
Copyright | |
19 other sections not shown
Common terms and phrases
adeno-associated virus adenovirus aliquot animal antibody assay blotting bone marrow cancer cassette cell culture cell line centrifuge chitosan concentration containing copolymers density dilution dishes DMEM dystrophin electroporation fibroblasts filter flask flow cytometry fluorescent gene delivery gene expression Gene Ther gene therapy genetic genome harvest HDAd hematopoietic human imaging Incubate injection Invitrogen lentiviral lentiviral vectors lipid liposome luciferase medium membrane Methods mg/ml mice Molecular monolayer mouse murine muscle nanoparticles Note optimal packaging cell pellet pipette plaques plasmid plasmid DNA plate polyplex preparation producer cells production protein protocol pseudotyped Qiagen reagent recombinant replication reporter gene Resuspend retroviral vector room temperature sample sequences shRNA siRNA solution stem cells sterile Subheading supernatant syringe therapeutic tissue culture titer transcription transduction transduction efficiency transfection transgene transgene expression tube tumor vial viral DNA viral vectors virus vitro vivo volume VSV-G µg/ml