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100 Grams sample acetic anhydride acetone acetylation acetylenic acetylenic compound acid chloride added adsorbent alcohol aldehyde alkali alkyl ammonium amount ampoule Anal analysis aniline apparatus aqueous Azo violet beaker benzene bisulfite blank boiling bromine burette Calculations carboxylic acid catalyst Chem color component concentrated curve described determined diazonium salt diluted dissolved disulfide electrode endpoint Erlenmeyer flask ethyl ethylene glycol-isopropanol excess functional group glacial acetic acid glass Grignard reagent hydrazine hydrochloric acid hydrogen hydrolysis hydroxyl groups indicator inorganic interfere iodine ketone membrane mercaptans mercuric methanol method milliliters minutes ml of water mole molecule NaOH nitrogen nitrous acid obtained organic compounds oxide peroxide phenol phthalate phthalic potassium iodide procedure pyridine quantitative react reaction flask sample containing secondary amine Siggia silver nitrate sodium hydroxide solution solvent stopcock stopper sulfate sulfuric acid temperature tertiary amine thiosulfate titanous chloride titrated with 0.1 titration tube unsaturated vinyl ether weighed