Culturing Nerve Cells

Front Cover
Gary Banker, Kimberly Goslin
MIT Press, 1998 - Medical - 666 pages
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Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.

The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.

Contributors: Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F. A. Finley, Gerald D. Fishbach, Beat H. Gä hwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Prö schel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T.Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.

  

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Contents

Getting Started
3
Types of Nerve Cell Cultures Their Advantages and Limitations
11
Primary Dissociated Cell Cultures
37
Transfecting Cultured Neurons
79
Characterizing and Studying Neuronal Cultures
113
Choosing the Right System
157
Neuronlike Cells Derived in Culture from PI 9 Embryonal Carcinoma
189
Culturing the Large Neurons of Aplysia
213
Mass Cultures and Microislands of Neurons from Postnatal Rat Brain
309
Rat Hippocampal Neurons in LowDensity Culture
339
Rat Striatal Neurons in LowDensity SerumFree Culture
371
Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial
395
Purification and Coculture of Identified Cell Populations
419
Organotypic Slice Cultures of Neural Tissue 441
461
Culture of Astrocytes Oligodendrocytes and thusProgenitor Cells
499
Tissue Culture Methods for the Study of Myelination
559

Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos
237
Cultures from Chick Peripheral Ganglia Ml
261
Culturing Mammalian Sympathoadrenal Derivatives
289
References
595
Index
659
Copyright

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