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Nucleic Acid Probes Labels and Labelling Methods
Denaturation Hybridization and Washing
5 other sections not shown
4x SSC/Tween acetate Amersham International amplification antibody Appendix avidin biotin biotin-labelled biotinylated blocking DNA Boehringer Mannheim buffer cell chromosome preparations cloned colloidal gold colour concentration confocal conjugated counterstaining coverslip DAPI denaturation detection reagents detection systems digoxigenin digoxigenin-labelled DNA polymerase DNA sequences DNA:DNA dNTP duplex electron microscopy emulsion enzyme ethanol Figure film filter fixative fluorescein fluorescence fluorochromes formamide gene Genet genomic grids horseradish peroxidase humid chamber hybridization experiments hybridization mix hybridization signal hybridization sites images incorporated Incubate interphase labelled nucleotide Laboratories light microscopy material metaphase method mRNA nick translation non-radioactively labelled non-specific nucleic acid nucleotides oligonucleotide plant precipitate primers probe hybridization reaction RNA probes RNA:RNA RNase Scale bar Section serum signal-generating system single-stranded sites of probe solution spread stains step stringency Table target sequences Texas red tissue total genomic DNA TrisHCI unlabelled vector viral visualization vitro transcription Wash slides wavelength