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0.2 mg sample acetone active compound active material added dropwise adjusted to pH alkaloid ammonium reineckate approximately Byers and Broquist carbonate and extracted CHCI3 extracts CHClg chloroform crude base fraction crude filtrate crystalline picrate crystals cultures Dowex 50 Dragendorffs reagent effluent Elution was carried equal volume equivalent weight evaporated to dryness evaporated under vacuum extracted three filtered beers Florisil column g animal gave a rating guinea pigs inoculated iodoplatinate reagent Ion Exchange Chromatography ion exchange resin isolated compound l2 hours l5 ml mg of material ml of chloroform ml of water mycelium ninhydrin obtained paper chromatography peak pH l0 Phosphotungstic Acid physostigmine picric acid positive spot precipitating agents procedure purification red clover residue redissolved residue weighed Rf's room temperature salivation saponin saturated aqueous solution shake flasks silicic acid column Slight activity solid sodium carbonate solvent spots were visible sprayed with iodoplatinate strip supernatant fluid test solution Thin Layer Chromatography toxic tube