Diagnostic Molecular Microbiology: Principles and ApplicationsDavid H. Persing Diagnostic Molecular Microbiology is the first major text to provide complete coverage of both the principles and applications of molecular diagnostic methods as they pertain to infectious diseases. Written and edited by leading international experts, this text provides both the theoretical and practical framework for understanding the powerful uses of nucleic acid amplification technologies and for applying these techniques to the rapid detection and characterisation of microbial pathogens (bacterial, viral, fungal, parasitic) in the clinical laboratory. The nine chapters in part 1: Principles summarise the basic theory underlying the emerging discipline of molecular diagnostics. The sixty–six protocols in part 2: Applications, offer proven applications of molecular diagnostic techniques for the diagnosis of infectious diseases. Written in the tradition of ASM′s other classic manuals, this book provides a valuable reference and teaching tool for any clinical microbiology laboratory. |
Contents
Molecular Typing Methods Bala Swaminathan | 26 |
Amplification Product Inactivation Methods David H Persing | 105 |
Sample Preparation Methods Larry Greenfield | 122 |
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Common terms and phrases
16S rRNA agarose gel aliquots amplification Amplification Control amplification product analysis annealing bacterial blood blot bromophenol blue cells Centrifuge Clin coli concentration containing culture cycles denaturation diagnosis digestion dilution disease DNA polymerase DNA probes dNTP EDTA enzyme ethanol ethidium bromide extraction fragments gene genomic human hybridization inactivation incubate infection isolates labeled laboratory lysis membrane method MgCl2 Microbiol microcentrifuge tube mixture Molecular NaCl nucleic acid Nucleotide Nucleotide sequence oligonucleotide oligonucleotide probes organisms pathogens patients PCR amplification PCR Detection PCR master mix PCR product pellet pipette plasmid polymerase chain reaction positive primer pair procedure proteinase proteinase K protocol Resuspend reverse transcriptase room temperature rRNA Sample Preparation sensitivity Sigma sodium solution specific sterile strains subtyping supernatant Taq polymerase target sequence TBE buffer techniques template thermal cycler tissue toxin Tris pH Tris-HCl pH viral virus volume vortex wash