Physical Properties of Biological Membranes and Their Functional ImplicationsCecilia Hidalgo This book was originated from a series of lectures given in a course on the physical properties of biological membranes and their functional implica tions. The course was intended to allow students to get acquainted with the physical techniques used to study biological membranes. The experience was valuable and we feel that a detailed description of the procedures used and of various examples of the results obtained allowed many students to become familiar with a theme that is not often part of regular courses on membrane physiology or biophysics. This book is designed as a tutorial guide for graduate students interested in understanding how physical methods can be utilized to study the proper ties of biological membranes. It includes first a detailed description of applications of physical techniques-such as X-ray fiber diffraction methods (Chapter 1), 2H and 13C NMR spectroscopy (Chapter 2), and calorimetry (Chapter 3)-in the study of the properties of lipid model membranes. A description of how to measure molecular mobility in membranes (Chapter 4) follows, and the book concludes with three chapters in which biological membranes are the subject of study. Chapter 5 deals with the acetylcholine receptor and its membrane environment; Chapter 6 discusses how fluorescence techniques can be applied in the study of the calcium ATPase of sarcoplasmic reticulum; and Chapter 7 explains how protein lipid interactions modulate the function of the sodium and proton pumps. |
Contents
1 | |
Geometrical Measurement of the Reflections and Information | 7 |
Conclusions | 17 |
Molecular Mobility in Membranes | 21 |
Lateral Diffusion | 35 |
Rotational Diffusion | 48 |
Chapter 5 | 68 |
A Single | 157 |
Dynamics of Acetylcholine Receptor and Lipids in the Membrane | 168 |
Chapter 6 | 177 |
Macromolecular Association | 196 |
ProteinProtein and ProteinLipid Interactions | 204 |
Chapter 7 | 211 |
Role of Sulfatides in the Proton Pump | 228 |
Other editions - View all
Physical Properties of Biological Membranes and Their Functional Implications Cecilia Hidalgo No preview available - 2013 |
Physical Properties of Biological Membranes and Their Functional Implications Cecilia Hidalgo No preview available - 2013 |
Common terms and phrases
2H NMR AChR acrylamide activity acyl chains angle anisotropy arylsulfatase atoms ATPase axis Barrantes binding Biochem Biochemistry Biol biological membranes Biophys Blume Ca2+ calculated calorimetry Chem chemical shift chemical shift anisotropy cholesterol components curves decrease density determined deuterium diffraction diffusion coefficient DMPA DMPC DMPE DPPC DPPE DSC scans electron emission enzyme equation excited Figure fluorescence intensity fluorophore fraction function gel phase head group heat hydrophobic increase indicate interactions kcal labeled line shapes lipid bilayers lipid molecules lipid-protein liquid-crystalline liquid-crystalline phase measured melittin mixtures mol¹ molecular molecules motion Na+,K+-ATPase observed obtained order parameter orientation ouabain phase transition phospholipids polarization powder patterns protein quencher quenching reaction rotational diffusion sarcoplasmic reticulum sarcoplasmic reticulum ATPase Seelig shown in Fig solution solvent specific spectra spectrum structure studies subunits sulfatide tensor transition enthalpy transition temperature tryptophan unit cell Verjovski-Almeida vesicles X-ray Zambrano