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Plasmid vectors for the regulated high level expression of euka
Amplification and stability of recombinant plas
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acid sequences activity amino acid analysis animal anti-HBs antigen assay bacterial Basel biol blot cell lines cellular characterization chromatography chronic coli containing culture Darai detection disease DNA fragments dot blot Eco RI electrophoresis enterotoxin Escherichia coli expression final product FLDV fusion protein genetic Geneva genome HBcAg HBsAg HBV infection hepatitis B surface hepatitis B virus herpesvirus host cell human hybridization immune immunogenic immunological insert Karger labelled laboratory lane liver Master Seed mice micelles molecular weight monoclonal antibodies nitrocellulose nucleic acid particles patients peptides plasmid polypeptides potency procedures produced by Recombinant protease purity R-DNA reactivity reagents Recombinant DNA Technology recombinant plasmid replication restriction enzymes SDS-PAGE sera serum HBV DNA Southern blot specific Standard strains studies substrates subunit surface antigen Switzerland synthetic peptides tac promoter techniques tests tion vaccine Vero cells viral DNA Virology virus DNA viruses vitro Western blotting Y-HBsAg yeast