Environmental Microbiology: A Laboratory Manual

Front Cover
Academic Press, 2005 - Science - 209 pages
0 Reviews
Section one: Basic Protocols. Experiment 1: Dilution and Plating of Bacteria and Growth Curves. Overwiew. Theory and Significance. Procedure.Triks of the Trade. Potential Hazards. Example Calculation of mean Generation time. Questions and Problems. Reference. EXPERIMENT 2: Soil Moisture Content Determination. Overview.Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Example Calculations. Questions and Problems. References. SECTION TWO: Examination of Soil Microorganisms Via Microscopic and Cultural Assays. EXPERIMENT 3: Contact Slide Assay. Overview.Theory and Significance. Procedure. Tricks of the Trade.. Potential Hazards. Questions and Problems. References.EXPERIMENT 4: Filamentous Fungi. Overview.Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards.. Calculations. Questions and Problem.References. EXPERIMENT 5: Bacteria and Actinomycetes. Overview.Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Questions and Problems. References.EXPERIMENT 6: Algae: Enumeration by MPN.Overview. Theory Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. References. SECTION THREE: Microbial Transformations and Response to Contaminants. Overview.Theory. Procedure. Tricks of the Trade. Potential Hazards. Calculations.Questions and Problems.References. EXPERIMENT 8: Dehydrogenase Activity of Soils. Overview. Theory. Procedure. Tricks of the Trade. Potential Hazards. Example Calculations. Questions and Problems. Reference. EXPERIMENT 9: Nitrification and Denitrification. Overview. Theory.Procedure. Tricks of the Trade. Potential Hazards. Assignment and Questions. References. EXPERIMENT 10: Enrichment and Isolation of Bacteria that Degrade 2,4-Dichlorophenoxyacetic Acid. Overview. Theory and Significance. Procedure; Tricks of the Trade. Potential Hazards. Questions and Problems.References. EXPERIMENT 11: Adaptation of Soil Bacteria to Metals. Overview.Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Questions and Problems. References. EXPERIMENT 12: Biodegradation of Phenol Compounds. Overview. Theory and Significance. Procedure. Potential Hazards. Calculations. Questions and Problem. References. EXPERIMENT 13: Assimilable Organic Carbon. Overview. Theory and Significance. Procedure. Tricks of the Trade. Calculations. Questions and Problems. References. EXPERIMENT 14: Biochemical Oxygen Demand. Overview. Theory and Significance. Procedure.Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. References. SECTION FOUR: Water Microbiology. EXPERIMENT 15: Bacteriological Examination of Water: The Coliform MPN Test. Overview.Theory and Significance. Procedure. Tricks of the Trade. Calculations. Questions and Problems. Reference. EXPERIMENT 16: Membrane Filter Technique. Overview. Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. Reference. EXPERIMENT 17: Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms. Overview. Theory and Significance.Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems.References. EXPERIMENT 18: Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces. Overview. Theory and Significance. Procedure.Tricks of the Trade. Questions and Problems. References. EXPERIMENT 19: Dection of Bacteriophages. Overview. Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. Reference. SECTION FIVE: Advanced Topics. EXPERIMENT 20: Detection of Enteric Viruses in Water. Overview. Theory and Significance.Procedure. Questions and Problems. References. EXPERIMENT 21: Detection of Waterborne Parasites.Overview. Theory and Significance.Procedure. Questions and Problems. References. EXPERIMENT 22: Kinetics of Disinfection. Overview. Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. Reference. EXPERIMENT 23: Aerobiology Sampling of Airborne Microorganisms.Overview. Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Calculations. Questions and Problems. Reference. EXPERIMENT 24: Detection and identification of Bacteria Via PCR and Subsequent BLAST Analysis of Amplified Sequences. Overview. Theory and Significance. Procedure. Tricks of the Trade. Potential Hazards. Questions and Problems. Reference. APPENDIX 1: Preparation of Media and Stains for Each Experiment. APPENDIX 2: Glossary.
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Examination of Soil Microorganisms Via Microscopic and Cultural Assays
17
Microbial Transformations and Response to Contaminants
59
Water Microbiology
113
Advanced Topics
147
Preparation of Media and Stains for Each Experiment
187
Glossary
197
Copyright

Other editions - View all

Common terms and phrases

Popular passages

Page 209 - USDA United States Department of Agriculture USEPA United States Environmental Protection Agency...

References to this book

All Book Search results »

About the author (2005)

Dr. Ian Pepper is currently a Professor at the University of Arizona. He is also Director of the University of Arizona, Environmental Research Laboratory (ERL) and the NSF Water and Environmental Technology (WET) Center. Dr. Pepper is an environmental microbiologist specializing in the molecular ecology of the environment. His research has focused on the fate and transport of pathogens in air, water, soils and wastes. His expertise has been recognized by membership on six National Academy of Science Committees and former memberships on an EPA FIFRA Science and Advisory Panel. Dr. Pepper is a Fellow of the American Association for the Advancement of Science, American Academy of Microbiology, the Soil Science Society of America, and the American Society of Agronomy. He is also a Board Certified Environmental Scientist within the American Academy of Environmental Engineers and Scientists. He is the author or co-author of six textbooks; 40 book chapters; and over 180 peer-review journal articles.

Dr. Charles P. Gerba is a Professor at the University of Arizona. He conducts research the transmission of pathogens through the environment. His recent research encompasses the transmission of pathogens by water, food and fomites; fate of pathogens in land applied wastes; development of new disinfectants; domestic microbiology and microbial risk assessment. He has been an author on more than 500 articles including several books in environmental microbiology and pollution science. He is a fellow of the American Academy of Microbiology and the American Association for the Advancement of Science. In 1998 he received the A. P. Black Award from the American Water Works Association for outstanding contributions to water science and in 1996 he received the McKee medal from the Water Environment Federation for outstanding contributions to groundwater protection. He received the 1999 Award of Excellence in Environmental Health from National Association of County and City Health Officials.

Bibliographic information