A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis

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Paul A. Krieg
John Wiley & Sons, Aug 15, 1996 - Medical - 445 pages
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Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques.

Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips.

A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms.

Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.
 

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Contents

CONTENTS
4
PATRICK PEzzoli The Immune Response Corporation 5935 Darwin Court Carlsbad
9
ISOLATION OF RNA FROM PLANT TISSUE
21
PAOLA PierANDREIAMALDI Instituto di Biologia Cellulare C N R Via Marx
43
ISOLATION OF POLYA+ RNA
54
ANALYSIS OF POLYSOMAL RNA
65
RNA BLOT ANALYSIS
83
ANALYSIS BY NUCLEASE PROTECTION
105
WUBo Li Life Technologies Inc 8717 Grovement Circle Gaithersburg MD 20877
225
APPLICATION OF DIFFERENTIAL DISPLAY TO CANCER
237
RNA FINGERPRINTING BY ARBITRARILY PRIMED PCR
251
A NEW METHOD FOR FULLLENGTH cDNA CLONING
273
AN RNA SUBTRACTION METHOD FOR LIBRARY
323
RADIOACTIVE IN SITU HYBRIDIZATION OF TISSUE
339
In SITU HYBRIDIZATION TO WHOLEMOUNT
357
WHOLEMOUNT IN SITU HYBRIDIZATION TO MOUSE
371

PRIMEREXTENSION ANALYSIS OF RNA
133
IN WITROSYNTHESIS OF mRNA
141
IN WITRO TRANSLATION OF CELLULAR AND SYNTHETIC
155
ANALYSIS OF MESSENGER RNA BY DIFFERENTIAL
223
TWOCOLOR WHOLEMOUNT IN SITU HYBRIDIZATION
381
ANALYZING PATTERNS OF ALTERNATIVE SPLICING
411
INDEX
441
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