A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis
Paul A. Krieg
John Wiley & Sons, Aug 15, 1996 - Medical - 445 pages
Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques.
Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips.
A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms.
Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.
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PATRICK PEzzoli The Immune Response Corporation 5935 Darwin Court Carlsbad
ISOLATION OF RNA FROM PLANT TISSUE
PAOLA PierANDREIAMALDI Instituto di Biologia Cellulare C N R Via Marx
ISOLATION OF POLYA+ RNA
ANALYSIS OF POLYSOMAL RNA
RNA BLOT ANALYSIS
ANALYSIS BY NUCLEASE PROTECTION
WUBo Li Life Technologies Inc 8717 Grovement Circle Gaithersburg MD 20877
APPLICATION OF DIFFERENTIAL DISPLAY TO CANCER
RNA FINGERPRINTING BY ARBITRARILY PRIMED PCR
A NEW METHOD FOR FULLLENGTH cDNA CLONING
AN RNA SUBTRACTION METHOD FOR LIBRARY
RADIOACTIVE IN SITU HYBRIDIZATION OF TISSUE
In SITU HYBRIDIZATION TO WHOLEMOUNT
WHOLEMOUNT IN SITU HYBRIDIZATION TO MOUSE
acetate amount amplification analysis annealing antibody aqueous phase bands buffer cap analog cells centrifugation cloning competitive PCR concentration containing cycles denaturing DEPC-treated detection dilution DNA probes EDTA efficiency electrophoresis embryos enzyme ethanol extraction filter formamide fractions full-length gene guanidinium homogenizer hybridization Incubate internal control template isolation of RNA isopropanol labeled lanes lysate method mg/mL microcentrifuge microfuge tube mRNA Northern blot nuclease protection assay Nucleic Acids nucleotide oligo-dT oligonucleotide PCR products PCR reaction phenol plasmid poly-A polysomes precipitation preparation primer procedure protection assays protein proteinase protocol purification quantitative reagents remove Resuspend reverse transcription ribonucleases ribosomal RNA isolation RNA pellet RNA polymerase RNA sample RNase protection RNase-free room temperature RT-PCR S1 nuclease sequence signal solution splicing staining step sterile stored supernatant synthesis synthetic mRNA target template tion tissue total RNA transcription reaction transfer translation vitro vitro transcription vortex wash yeast