A Laboratory Guide to the Mammalian Embryo

Front Cover
David K. Gardner, Michelle Lane, Andrew J. Watson
Oxford University Press, Jan 8, 2004 - Science - 432 pages
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This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the Mouse Embryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.
 

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Contents

1 Follicle Development in Vitro
3
2 Preparation of Gametes in Vitro Maturation in Vitro Fertilization and Embryo Recovery and Transfer
24
3 Culture of the Mammalian Preimplantation Embryo
41
4 Assessment of Preimplantation Embryo Development and Viability
62
5 Micromanipulation of Gametes
76
Biopsy
99
pH and Calcium
125
8 Assessment of Nutrient Uptake Metabolite Production and Enzyme Activity
139
14 Relative mRNA Transcript Abundance in Early Embryos by Reverse TranscriptionPolymerase Chain Reaction
225
15 Characterization of Novel Genes during Early Development by Application of Differential Display RTPCR
237
16 TwoDimensional Protein Gel Database Analysis of Embryos Oocytes and OocyteAssociated Granulosa Cells
247
17 Antisense Disruption of Gene Expression in the Preimplantation Embryo
258
18 Gene Subtraction and Analysis
270
19 Analysis of Apoptosis in the Preimplantation Embryo
279
20 Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo
298
21 Human Embryonic Stem Cells
313

9 Metabolic Pathway Activity
154
10 Confocal Imaging of Structural Molecules in Mammalian Gametes
165
11 Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis
184
12 Assessment of Ploidy Telomere Length and Telomerase Activity in Oocytes and Embryos
193
13 Microscale RNA Isolation from Mammalian Embryos
217
22 Spermatogonial Transplantation
334
23 Somatic Cell Nuclear Transfer
352
Slow Freezing Vitrification of Embryos
375
Index
391
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