A Practical Guide to Basic Laboratory Andrology
Lars Bj÷rndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen
Cambridge University Press, Apr 1, 2010 - Medical
This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Equipment required for a basic andrology laboratory
Recommendations on the application of CASA technology in the analysis of spermatozoa
Example laboratory and report forms
Comparison of replicate counts
Other editions - View all
abnormal acrosome reaction aﬀect Androl andrology andrology laboratory antibodies antisperm antibodies ASABs assay azoospermia Calibration capacitation CASA cells centrifugation cervical mucus chromatin clinical container counted coverslip cryobank cryopreservation deﬁned deﬁnition diﬀerent dilution Disposable materials donor eﬀects ejaculate epididymis equipment evaluation factor Fertil Steril ﬁeld ﬁnal ﬁrst ﬂuid fructose gametes hemocytometer Hum Reprod human semen human sperm human spermatozoa hyperactivation ICSI identiﬁed infertility insemination Interpretation guidelines leukocytes liqueﬁed magniﬁcation male method microscope Mortimer motile spermatozoa normal number of spermatozoa oocyte Pasteur pipettes patient pellet pipette procedure progressively motile prostatic quality control Reagents resuspend risk semen analysis semen sample seminal plasma seminal vesicles signiﬁcance slide smears solution speciﬁc specimen Sperm Buﬀer sperm capacitation sperm chromatin sperm concentration sperm DNA sperm morphology sperm motility sperm preparation spermatogenesis staﬀ staining standard storage straws subfertility suﬃcient techniques temperature total number tube values vitro volume zinc zona pellucida