A Practical Guide to Basic Laboratory Andrology

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Cambridge University Press, Apr 1, 2010 - Medical
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This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
 

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Contents

Introduction
1
Basic physiology
5
Basic semen analysis
33
Extended semen analysis
77
Sperm function tests
113
Tests of spermcervical mucus interaction
147
Sperm preparation
167
Sperm cryobanking
189
Reproductive toxicology
257
Reference values
261
Equipment required for a basic andrology laboratory
277
Supply companies
287
Recommendations on the application of CASA technology in the analysis of spermatozoa
291
Home testing
295
Example laboratory and report forms
297
Comparison of replicate counts
305

Preparation of surgically retrieved spermatozoa
219
Quality management and accreditation
227
Risk management
249
Safety
313
Index
323
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About the author (2010)

Lars Bj÷rndahl is Consultant, Center for Andrology and Sexual Medicine, Clinic of Endocrinology, Department of Medicine, Karolinska University Hospital and Karolinska Institutet, Huddinge, Stockholm, Sweden.

David Mortimer is the President and co-owner of Oozoa Biomedical Inc., West Vancouver, British Columbia, Canada.

Christopher L. R. Barratt is Professor of Reproductive Medicine, University of Dundee, Scotland.

Jose Antonio Castilla is Director of Andrology and Embryology Laboratory, Hospital Virgen de las Nieves, Spain and Medical Director of Sperm Bank CEIFER, Granada, Spain.

Roelof Menkfeld is Head Specialist Scientist and Director of the Andrology Laboratory, Department of Obstetrics and Gynaecology, Tygerberg Academic Hospital and University of Stellenbosch, Tygerberg, South Africa.

Ulrik Kvist is Associate Professor of Andrology, Director of Andrology Laboratory, Center for Andrology and Sexual Medicine, Karolinska University Hospital and Karolinska Institute, Sweden.

Juan G. Alvarez is Scientific Director, Institut Marques, Barcelona, Spain.

Trine B. Haugen is Professor, Faculty of Health Sciences, Oslo University College, Norway.

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