A Practical Guide to the Study of Calcium in Living Cells
Academic Press, Apr 25, 1994 - Science - 368 pages
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
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acid activity aequorin affinity BAPTA binding Biochem Biol biological Biophys buffer Ca”-free Ca”-selective Calcium Green calculated calibration camera cDNA Cell Biology cells cellular changes channels chelators Chem compounds concentration confocal confocal microscopy cyclopiazonic acid cytoplasm cytosolic deblurring described detection detector dichroic mirror digitonin DM-nitrophen effect eggs EGTA electrode emission ester excitation experimental fluorescence free calcium in-focus inhibitors injected intensity intracellular intracellular calcium ion-selective ionic strength ionomycin ionophore laser levels ligand light loaded luminescence measured medium membrane method microinjection micropipette microscope Molecular muscle Nature London neurons nitr oocytes optical organelles out-of-focus patch photolysis photon Physiol pipette pixel potential probe processing protein pump ratio ratiometric receptor recombinant aequorin release response reticulum sarcoplasmic reticulum scanning Section sensitivity SERCA signal silane SNARF-1 solution spatial technique temperature thapsigargin tion Tsien values vibrating vitro voltage wavelength Xenopus oocytes Zucker