A Stitch in Time
Hypermethylated In Cancer 1 (HIC1) gene was identified as a potential tumour suppressor but its physiological role has remained enigmatic. This work (master thesis) provides insights into the biological relevance of HIC1 not only in the context of Wnt signalling. The book contains summary of data on the Wnt signalling pathway and HIC1 available in the literature (current of April 2008) and very broad yet consistent set of laboratory approaches and detailed manuals. These methods can be very helpful to students interested in molecular biology. The whole work is followed by thorough discussion of the presented results. Most interestingly, one chapter of the book describes detailed strategy for generating gene targeted mouse. This is exemplified by production of two strains: (1) a strain for conditional inactivation of Hic1 gene using Cre/loxP system and (2) so-called reporter mouse strain that expresses the fluorescent protein from endogenous Hic1 promoter. A separate file containing the author s lecture Generation of conditional knock-out mouse step by step is included to provide a comprehensive overview of the research method that has pervaded all fields of contemporary biology.
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LibraryThing ReviewUser Review - LindaGail - LibraryThing
Interesting read about a fund raiser/event planner who caught her husband having an affair, she has had her stomach stapled and has lost 150 pounds, her grown children are in their late 20's and won't leave the nest, and she is a fairly new born again Christian. Read full review
A stitch in timeUser Review - Not Available - Book Verdict
Losing 150 pounds, separating from her husband, and becoming a Christian all in one year have transformed middle-aged Southern Californian Dee Decker. Can Dee learn how to handle her new life, especially when a job offer changes everything? First novelist Bottke lives in Minnesota. Read full review