A Study on Aspergillus Flavus
GRIN Verlag, 2011 - 60 pages
Technical Report from the year 2011 in the subject Biology - Micro- and Molecular Biology, The University of Mysore (-), course: Biotechnology, language: English, abstract: Aspergillus flavus is the most widely known species of the genus Aspergillus which is known as a species in 1809 and first reported as a plant pathogen in 1920. Many fungi produce mycotoxin, a toxic secondary metabolite. Most A. flavus produces aflatoxin, a type of mycotoxin that is harmful for human, animal, and plant health. Characterization study of this saprophyte is important for the management of various human, animal, and plant diseases as well for surveillance, and other epidemiological study. The study focused on 3 subjects, morphological and physiological study, enzyme activity, and aflatoxin production from ground nut seeds collected from all over India. The growth of this fungus was compared in 4 different media for morphological study and in 4 different temperature on PDA media for physiological study. For enzyme activity, amylolytic, proteolytic, lipolytic, cellulolytic, pectinolytic and catalytic activity were determined. The presence of aflatoxin was performed using thin layer chromatography method. Among 12 isolates, amylase activity was shown by only 12 isolates and there is no clear zone in the remaining 8 isolates. Five isolates did not produce any protease among the 20 tested. Where as 18 isolates were positive for pectin methyl production. In case of lipase, 3 isolates did not show any white precipitation and all other isolates showed white precipitate due to the activity of lipase. Cellulose activity was very low in all the tested isolates and only 2 (AF7 and AF14) isolates exhibited the activity of cellulose in culture plates. Specific activity of catalase was calculated for all the isolates and all the isolates showed varied level of activity. The highest activity (210 mg/ml) was recorded by AF3 isolate and the least
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15 Green 30 Green 40 Opaque Brown 6.1 Cotton Entire 6.5 Powdery Wavy activity of catalase activity shown AFB1 AFB2 aflatoxin B1 agar Amylase Applied Botany aspergilloma Aspergillus flavus isolates brown centre PDA catalase catalase activity cellulolytic cellulose characteristics of Aspergillus characterization of Aspergillus clear zone Colony Colony Colony Cotton Wavy Creamish white 4.6 Department of Studies distilled water edge Cream 5.0 edge Creamish white electrophoresis Entire More brown enzyme fungal fungal growth fungus Green with white h AFPA Hedayati incubation infection isoform isolate in culture isolates showed lipase Lisa Nathalie Tantio medium used contained mg/ml parasiticus pathogens pectin pectinase plant cell wall potato dextrose Powdery Wavy Less presence of aflatoxin produce aflatoxin protease Red 6.2 Cotton secondary metabolite sporulation Studies in Applied toxigenic University of Mysore water 1 litre white 4.6 Powdery white edge Cream white edge Creamish Yellow with white YESA White Yellow