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EFFECT OF MEDIUM ON THE PRODUCTION OF
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120 minutes active bactericidal aerobic conditions agar extracts agar plates anaerobic antibiotics Bacillus subtilis bacterial lawns bactericidal factor activity bactericidal factor preparation bactericidal factor production bactericidal factor treated bactericidal substance Bio-gel P-2 Brain heart infusion broth assay cells were exposed colicin concentration decrease described in Materials detected determined diluted DS-1912-65 bactericidal factor DS-l9l2-65 bactericidal effect of bactericidal elution profiles enzymes Escherichia coli exposed to bactericidal extracellular Figure group A strains group A streptococci growth hydrogen peroxide icidal factor inactivated bactericidal factor inhibitory activity inhibitory substance ion exchange columns killing activity lawn mammalian cells Materials and Methods membrane minimal medium monkey kidney cells Ninhydrin nisin percent plate assay produced by group producer cells producer strain protein proteose peptone Purified bactericidal factor S716 bactericidal factor sensitive cells Sephadex Staphylococcus aureus Streptococcus pyogenes Table temperatures tested tion Todd Hewitt Trichophyton rubrum tyrosine uninoculated viable count zones of inhibition