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Simplified basic theory of electron microscopy
The processing of tissues
The preparation of ultrathin sections for electron
8 other sections not shown
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Academic Press acetone acid aldehyde alignment appear Araldite astigmatism Autoradiography block buffer carbon Cell Biology cent centre chemical clean collodion components condenser contamination contrast Cytochemistry Cytology cytoplasm dehydration developed diffraction diffusion pump distilled water electron beam electron micro electron microscopy electron stains embedding material embedding medium emulsion enzyme Epon ethanol evaporation exposure filament film fixation fixative fluorescent screen focus focusing formaldehyde Formvar freezing fringe glass glutaraldehyde grids high resolution high voltage Histochemistry and Cytochemistry illumination inch Journal of Cell Journal of Histochemistry Journal of Ultrastructure knife edge laboratory lead citrate lenses light microscope lipid magnification membranes methacrylate method microtome molecules objective lens obtained organelles osmium tetroxide photographic plate produced protein reaction removed replica Rinse routine scanning Sjostrand slide specimen holder structure surface techniques temperature thickness thin tion tissue ultramicrotome Ultrastructure Research ultrathin sections uranyl acetate usually vacuum vary York and London