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Simplified basic theory of electron microscopy
The processing of tissues
The preparation of ultrathin sections for electron
8 other sections not shown
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Academic Press acetone acid aldehyde alignment Araldite astigmatism Autoradiography block buffer carbon Cell Biology cent centre chemical clean collodion column components condenser contamination contrast Cytochemistry Cytology cytoplasm dehydration developed diffusion pump distilled water electron beam electron micro electron microscopy electron stains embedding material embedding medium emulsion enzyme epoxy resins ethanol evaporation exposure filament film fixation fixative fluorescent screen focus focusing formaldehyde Formvar freezing glass glass knife glutaraldehyde grids high resolution high voltage Histochemistry and Cytochemistry illumination inch Journal of Biophysical Journal of Cell Journal of Histochemistry Journal of Ultrastructure knife edge laboratory lead citrate lenses light microscope light microscopy lipid magnification membranes methacrylate method micrographs microtome molecules objective lens obtained organelles osmium tetroxide photographic plate protein reaction removed replica Rinse routine Sjostrand slide specimen holder structure surface temperature thickness tion tissue ultramicrotome ultrathin sections uranyl acetate usually vacuum vary York and London