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75 per cent absolute alcohol ammonia animal aorta bath blood blood-vessels camel-hair brush cannula carmine gelatine carrot cartilage celloidin centimetres chloroform chromic acid colour cord cork cornea cover cover-glass DEFINITIONS OF TERMS diaphragm dissolved distilled water drop droplet edge embedding epithelium eye-piece filoselle filter flame forceps fresh frog gelatine gland glass tubing glycerine glycerine jelly gold gum and syrup half inch injected keep kidney knife Lastly lens lenses liver logwood mass melted melting-point membrane method methylated spirit microscope microtome minutes moist chamber mount in balsam mounted in glycerine mucilage mucous nerve nozzle object olfactory epithelium paraffin picric picric acid picro-carmine piece of tissue pipette pound Ranvier's alcohol removed salt-solution saucer sections shallow cell silver silver-solution slide spectra spirit-lamp student surface syringe taken tap-water thin turpentine twelve hours twenty-four hours wash watch-glass wax and oil whilst Wolff's bottle xylol xylol balsam
Page 23 - To do this, put a stage-micrometer (which is a glass slide ruled in the centre with lines ^ and ^fa millimeter apart) under the microscope in such a manner that the lines run from left to right (the microscope must not be inclined). Focus them exactly. Put a piece of white card on the table at the right of the microscope. Look through the instrument with the left eye, keeping the right eye open. The lines of the micrometer will appear projected upon the paper. Mark their apparent distance with pencil...
Page 24 - This scale-card will serve for the measurement of any object without the further use of the micrometer. To measure an object, place the scale-card upon the table to the right of the microscope and view the object with the left eye, keeping the right eye open. The object appears projected upon the scale, and its size in — or j!|5 of a millimeter can be read off.
Page 144 - The height to which a column of liquid will rise in a tube which it wets is inversely proportional to the diameter of the tube.
Page 115 - C) or of y-hexachlorocyclohexane of at least 99% purity (lindane) are made up in a mixture of equal parts of absolute alcohol and ether in which 0.2% celloidin has been dissolved.
Page 195 - MATERIAL. two copper rectangular straps riveted to the cardboard. A rectangular piece is cut out of the middle of the horizontal half, and a glass slip put in between the cardboard and the copper straps. Two slits in the upright half 1 cm. apart admit a length (12 cm.) of broad tape. The frog sits quietly for half an hour at a time upon this contrivance with or without whiffs of chloroform.
Page 16 - ... all these images cannot represent the true structure. If, however, we are pretty sure that we have all the characteristic spectra, and their position and relative intensity can be calculated, then the resultant image can also be calculated ; and so far as all the spectra are included it will FIG.
Page 17 - As we have found before that the finer the grating the more widely deflected are the diffracted spectra, we can now readily understand how, as regards minute structure especially, collection of the widest possible angular field of rays from the object is a point of the utmost importance for correct delineation, quite irrespective of greater illumination ; and it is in this respect that immersion objectives have such an enormous advantage. Of course these considerations only apply to structure of...
Page 115 - ... adhering to the slide. The cigarette-paper prevents the fibers of the filter-paper from adhering to the section and marring its appearance. 6. Cover the section sticking to the slide with absolute alcohol for about thirty seconds and drain. 7. Flow over the section and the adjacent surface of the slide a very thin solution of celloidin in equal parts of absolute alcohol and ether. Drain off immediately. The celloidin should form a coating so thin as to be invisible. 8. Flood the slide with ninety-five...
Page 11 - If between the reflecting-mirror and the stage of the microscope we interpose a very small opening in the diaphragm, and on the stage lay a grating of ruled lines, on removing the eye-piece and looking down the tube we observe a series of images of the aperture, like Fig. 4, all circular in homogeneous light, but the outer ones consisting of spectra in white light. The small pencil admitted through the diaphragm is
Page 197 - ... below the muscle, and keeping it well stretched, pour a little '5 pc osmic acid over it. In a minute or so the muscle will be fixed (the connective tissue surrounding the muscle may be torn away with fine forceps but in doing this there is considerable danger of injuring the muscle and its nerve) ; cut it out, being careful to cut the upper end as close as possible to the skin ; place it in osmic acid for 15 to 30 minutes, wash with water.