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RESULTS AND DISCUSSION
The Inhibition of Ascorbic Acid Destruction
A Kinetic Study of the Degradation
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2,4-dinitrophenylhydrazine 8-hydroxyquinoline absorption spectra Acid in Phosphate acid was incubated aliquots amount anaerobically appeared aqueous solution ascorbic acid Ascorbic acid incubated ascorbic acid present barely visible barely barely visible trace Biochem Biol catalyst catalyze Chea Chem chloroform chromatographic color complex compounds concentration containing copper copper-catalyzed oxidation degradation of ascorbic degradation of dehydroascorbic degradation products dehydroascorbic acid dehydroascorbic and diketogulonic Destruction of Dehydroascorbic detected diketogulonic acid dismutation distilled water dithizone EDTA ethyl acetate extracted Fe++ Fe+++ ferric chloride ferrous FIGURE flask formed heavy metal hydrogen peroxide hydroxamic acid hydroxyl hydroxylamine hydroxyproline incubation period iron-catalyzed oxidation Methanol-acetone-acetic acid method methyl model system molecular oxygen moles per liter optical density oxidation of ascorbic paper phosphate at pH phosphate buffer presence of phosphate procedure proline radicals reaction mechanism reagent reduced reported Rf values samples sodium phosphate Solvent Systems spots sprayed studies substrate trace barely visible trace heavy trace visible barely visible Vycor Zilva