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95 per cent Absolute alcohol Acetone Agricultural Chemistry alcohol residue amounts of vitamin antiscorbutic properties antiscorbutic vitamine autoclave Benzene Biol BIOLOGICAL CHEMISTRY Vol Butyl alcohol casein cent alcohol cent ethyl alcohol Char Chem Chloroform cod liver oil daily 20 gm desiccated orange juice distilling Drummond E. B. HART eaten daily 20 ethyl acetate Ethyl ether evaporated extract experimental fan at room four animals guinea pig Harden and Zilva heated grain ration heated ration insolubility JOURNAL OF BIOLOGICAL kilo LEPKOVSKY and MARIANA Madison Merck preparation Merck U. S. P. Methyl alcohol ncor oatmeal Parsons Petroleum ether PlUB prepared by treating Profeeeor ration supplemented rats raised Roll Alfa room temperature SAMUEL LEPKOVSKY scorbutic ration scorbutic vitamine scurvy second generation experiments second generation rats SOLUBILITY FROM DESICCATED soluble in 80 solvents tried soon after weaning Steenbock stock ration theeie united extracts vitamine of desiccated WAVERLY PRESS BALTIMORE yeast
Page 243 - Published with the permission of the Director of the Wisconsin Agricultural Experiment Station, Madison.
Page 244 - EXPERIMENTAL. In our first experiments the orange juice was squeezed from fresh oranges, pressed through cheese-cloth, and 300 cc. were poured onto sheets of filter paper and dried before a fan at room temperature (about 20°C.). The paper was then broken up, placed in flasks, and subjected to the action of the solvent in portions of 200 to 300 cc. at room temperature for a period of 2 days. This was repeated twice—the solvent always being 'filtered aftef contact with the paper.
Page 257 - This criterion, applied to their limited experimental data, has been'aptly criticized by Osborne and Mendel (4) who pointed out that additional amounts of vitamin B known to be contained in these juices might have brought about the same result. There is no doubt much justice in this criticism for as Osborne and Mendel state: * Published with the permission of the Director of the Wisconsin Agricultural Experiment Station.
Page 247 - ... the antiscorbutic vitamine of desiccated orange juice is insoluble in all of the solvents tried, with the exception of absolute, 95 and 80 per cent ethyl alcohol, and methyl alcohol. This confirms our CHART 2. The insolubility of the antiscorbutic vitamine in anhydrous ethyl ether is shown in this chart. All the animals succumbed to scurvy.
Page 262 - This was determined by their fall hi weight and degree of involvement of the wrists. When all question of failure from scurvy was removed liver as prepared by Parsons was administered in graduated amounts. Chart II shows the results obtained when liver from first and second generation rats was fed daily in 1 and 3 gm.
Page 243 - ... and filtered orange juice and its presence in the water extract of fresh plant tissue. Whether it would be soluble in water in the absence of other compounds occurring with it in such materials as orange juice, fresh green tissue, etc., has not so far as we are aware been determined. Hess and Unger 1 have reported that it is soluble in 80 per cent alcohol.
Page 260 - A grain ration to the exclusion of milk or alfalfa supplements was used as grains are admittedly low in their content of vitamin C. This was heated in an attempt to reduce further the vitamin C content. As the chart shows this turned out to be prejudicial to the wellbeing of the rats. Rats 8078, 8079, 8083, 8086, and 8087 were litter mates and so were Rats 8080,8081,8082,8084,8085,8088,and 8089.
Page 248 - ... induce recovery from scurvy and continued growth. Even in the case of the ethyl alcohol of varying concentrations not all of the vitamine was removed by the volume of solvent used, as shown in...
Page 260 - ... from drinking tubes. They were sacrificed for their livers when they weighed from 220 to 330 gm., average 279 gm. The interval spent on the ration ranged from 103 to 114 days, average 108 days. In the second generation experiments no accurate record of ages and weights was kept. Though their Gme.
Page 244 - ... alcohol. All of the other solvents failed completely. The animals succumbed to scurvy in 5 to 7 weeks, although had they eaten daily 20 gm. of the ration (a conservative consumption for a normal guinea pig of 300 gm. weight) they would have received the equivalent of 6 cc. of desiccated orange juice.