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METHODS AND MATERIALS
TOTAL HAEMOCYTE COUNTS
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acidophilic adhering adult stage amaranth dye average basophilic Blatta orientalis cell dilution method chromatin circulating cells circulating haemocytes classification coagulation cockroach cuticle cytes cytoplasm decrease degenerating cells desert locust differential counts differential grasshopper differentialis dium dividing haemocytes early stadia ecdysis Entomol excretion fifth granular haemocytes haemo haemocoele haemocytopoietic haemolymph volume determination haemolymph volume percent Hrdy increase in haemolymph indicate individuals insects instar inulin Jones larvae Levenbook 1958 Malpighian tubules microliters microns minutes mitosis mitotically dividing cells naked nuclei nucleocytes number of cells number of circulating number of haemocytes nymphal stadia nymphal stages nymphs orthopteran Periplaneta americana phase contrast microscope pipette planchet plasmatocytes postembryonic sample Schistocerca gregaria sixth stadium species spheroidocytes staining studies Table Tauber technique THC's third day third stadium tissues total count total haemocyte count total number trend Type unfixed haemolymph unit weight vacuoles volume per unit Wheeler Yeager and Munson Zool