A Study of the Interaction of Macromolecules with Erythrocytes |
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0.01 N Cysteine 0.1 N Cyanide agglutination AGGLUTINATION TITER antibody antigens binding blood bond carbazole cation cell populations cells treated centrifuged Precipitate COMPLEMENT TITRATION compound concen CRUDE PAPAIN decrease diluted distilled water EFFECT OF ACTIVATOR EFFECT OF DIGESTION Effect of papain enzyme ENZYME CONCENTRATION experiments Fåhraeus Feigen and Campbell Figure galacturonic acid gelatin glycerol pectate GUINEA PIG SERUM hemolysis heterogeneity hydrogen bonding hydroxybenzoic acid increase incubation inhibited interaction isoelectric point isomers KC1-NaCl Krogh equation lyophilized melting point meta method mg/ml MILK CLOTTING milliliter molecular weight molecules NaCl non-activated non-specific observed ortho ortho-hydroxybenzoate OSMOTIC FRAGILITY osmotic pressure PAPAIN FRACTIONS pectin percent plasma plot present protein pure papain rabbit erythrocytes reaction reagent red cell reduced relationship samples sedimentation rate showed sodium salicylate stored at 2°C studies Supernate suspension stability Table tested tion titer tration trypsin tubes values veronal buffer viscosity volume wash solution